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Product Info Summary
| SKU: | A06850-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-DP2/TFDP2 Antibody Picoband™
SKU/Catalog Number
A06850-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DP2/TFDP2 Antibody Picoband™ catalog # A06850-3. Tested in WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-DP2/TFDP2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06850-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human DP2/TFDP2, identical to the related mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06850-3 is reactive to TFDP2 in Mouse, Rat
Applications
A06850-3 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
49 kDa
Calculated molecular weight
49.236kDa
Background of DP2
Transcription factor Dp-2 is a protein that in humans is encoded by the TFDP2 gene. The gene is a member of the transcription factor DP family. The encoded protein forms heterodimers with the E2F transcription factors resulting in transcriptional activation of cell cycle regulated genes. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TFDP2 using anti-TFDP2 antibody (A06850-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFDP2 antigen affinity purified polyclonal antibody (Catalog # A06850-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TFDP2 at approximately 49 kDa. The expected band size for TFDP2 is at 49 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TFDP2 (Source: Uniprot.org, NCBI)
Gene Name
TFDP2
Full Name
Transcription factor Dp-2
Weight
49.236kDa
Superfamily
E2F/DP family
Alternative Names
DKFZp313O196; DP2Dp-2; E2F dimerization partner 2; FLJ39131; transcription factor Dp-2 (E2F dimerization partner 2); transcription factor Dp-2 TFDP2 DP2 transcription factor Dp-2 transcription factor Dp-2|transcription factor Dp-2 (E2F dimerization partner 2)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TFDP2, check out the TFDP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TFDP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DP2/TFDP2 Antibody Picoband™ (A06850-3)
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No publications found for A06850-3
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